应用
Brilliant Blue G is used in analytical biochemistry dye and as a P2X7 antagonist. It is also used in protein staining following gel electrophoresis. It is used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery. It is useful for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualized during staining. It has been used in the Bradford dye-binding protein assay. A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents.

备注
E(m) (595nm, H{2}O) >36,300

基本信息

MDL
MFCD00078482

EINECS
228-058-4

分子式
C₄₇H₄₈N₃NaO₇S₂

分子量
854.04

灵敏度
Ambient temperatures.

形态
Powder

溶解性
Soluble in water (50 mg/ml) and ethanol (40 mg/ml).

安全信息

TSCA
是